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Database Commons - HuSiDa

HuSiDa

Citations: 43

z-index 0.00

Short name HuSiDa
Full name human siRNA database
Description The human siRNA database (HuSiDa) provides sequences of published functional siRNA molecules targeting human genes and important technical details of the corresponding gene silencing experiments, including the mode of siRNA generation, recipient cell lines, transfection reagents and procedures and direct links to published references (PubMed).
URL http://www.human-siRNA-database.net
Year founded 2004
Last update & version     
Availability Not Available
University/Institution hosted Charite - University Medicine Berlin
Address Charité, Universitätsmedizin-Berlin, Germany
City Berlin
Province/State
Country/Region Germany
Contact name Matthias Truss
Contact email Matthias.Truss@Charite.de
Data type(s)
Major organism(s)
Keyword(s)
  • siRNA
Publication(s)
  • HuSiDa--the human siRNA database: an open-access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells. [PMID: 15608157]

    Matthias Truss, Maciej Swat, Szymon M Kielbasa, Reinhold Schäfer, Hanspeter Herzel, Christian Hagemeier
    Nucleic acids research 2005:33(Database issue)
    43 Citations (Google Scholar as of 2016-01-19)

    Abstract: Small interfering RNAs (siRNAs) have become a standard tool in functional genomics. Once incorporated into the RNA-induced silencing complex (RISC), siRNAs mediate the specific recognition of corresponding target mRNAs and their cleavage. However, only a small fraction of randomly chosen siRNA sequences is able to induce efficient gene silencing. In common laboratory practice, successful RNA interference experiments typically require both, the labour and cost-intensive identification of an active siRNA sequence and the optimization of target cell line-specific procedures for optimal siRNA delivery. To optimize the design and performance of siRNA experiments, we have established the human siRNA database (HuSiDa). The database provides sequences of published functional siRNA molecules targeting human genes and important technical details of the corresponding gene silencing experiments, including the mode of siRNA generation, recipient cell lines, transfection reagents and procedures and direct links to published references (PubMed). The database can be accessed at http://www.human-siRNA-database.net. We used the siRNA sequence information stored in the database for scrutinizing published sequence selection parameters for efficient gene silencing.

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Tags

RNA
Homo sapiens
siRNA

Record metadata

  • Created on: 2015-07-11
  • Curated by:
    • Lina Ma [2016-04-13]
    • Chunlei Yu [2016-03-31]
    • Mengwei Li [2016-02-20]
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