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Database Commons - qPrimerDepot

qPrimerDepot

Citations: 105

z-index 4.87

Short name qPrimerDepot
Full name qPrimerDepot
Description qPrimerDepot is a primer database for quantitative real time PCR
URL http://primerdepot.nci.nih.gov/
Year founded 2007
Last update & version 2007-06-25    v1.0
Availability Free to all users
University/Institution hosted National Cancer Institute
Address MD 20892-4605
City Bethesda
Province/State MD
Country/Region United States
Contact name Kevin Gardner
Contact email gardnerk@mail.nih.gov
Data type(s)
Major organism(s)
Keyword(s)
  • primer
  • real time PCR
Publication(s)
  • qPrimerDepot: a primer database for quantitative real time PCR. [PMID: 17068075]

    Wenwu Cui, Dennis D Taub, Kevin Gardner
    Nucleic acids research 2007:35(Database issue)
    105 Citations (Google Scholar as of 2016-05-01)

    Abstract: Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

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200 OK2016-01-29
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200 OK2016-01-25
200 OK2016-01-24
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200 OK2016-01-17
200 OK2016-01-15
200 OK2016-01-13
200 OK2016-01-11
200 OK2016-01-10
200 OK2016-01-08
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200 OK2016-01-04

Tags

DNA Expression RNA
primer real time PCR

Record metadata

  • Created on: 2015-08-10
  • Curated by:
    • Lina Ma [2016-04-11]
    • Lin Xia [2016-03-28]
Stats