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Database Commons - ICG

ICG

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Short name ICG
Full name A knowledgebase of Internal Control Genes for RT-qPCR normalization
Description ICG is a wiki-based knowledgebase of internal control genes (or reference genes) for RT-qPCR normalization in a variety of species. Based on community curation, ICG harnesses collective intelligence to integrate a comprehensive collection of internal control genes curated from a large volume of literatures and provides appropriate internal control genes corresponding to specific experimental conditions for both model and non-model organisms.
URL http://icg.big.ac.cn/index.php/Main_Page
Year founded 2017
Last update & version 2017-10-03    1.0
Availability Free to all users
University/Institution hosted BIG Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences
Address No.1 Beichen West Road, Chaoyang District, Beijing 100101, China
City Beijing
Province/State Beijing
Country/Region China
Contact name ZhangZhang
Contact email ZhangZhang(AT)big.ac.cn
Data type(s)
Major organism(s)
Keyword(s)
  • RT-qPCR normalization
Publication(s)
  • ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization. [PMID: 29036693]

    Jian Sang, Zhennan Wang, Man Li, Jiabao Cao, Guangyi Niu, Lin Xia, Dong Zou, Fan Wang, Xingjian Xu, Xiaojiao Han, Jinqi Fan, Ye Yang, Wanzhu Zuo, Yang Zhang, Wenming Zhao, Yiming Bao, Jingfa Xiao, Songnian Hu, Lili Hao, Zhang Zhang
    Nucleic acids research 2017:
    Citation (to be updated)

    Abstract: Real-time quantitative PCR (RT-qPCR) has become a widely used method for accurate expression profiling of targeted mRNA and ncRNA. Selection of appropriate internal control genes for RT-qPCR normalization is an elementary prerequisite for reliable expression measurement. Here, we present ICG (http://icg.big.ac.cn), a wiki-driven knowledgebase for community curation of experimentally validated internal control genes as well as their associated experimental conditions. Unlike extant related databases that focus on qPCR primers in model organisms (mainly human and mouse), ICG features harnessing collective intelligence in community integration of internal control genes for a variety of species. Specifically, it integrates a comprehensive collection of more than 750 internal control genes for 73 animals, 115 plants, 12 fungi and 9 bacteria, and incorporates detailed information on recommended application scenarios corresponding to specific experimental conditions, which, collectively, are of great help for researchers to adopt appropriate internal control genes for their own experiments. Taken together, ICG serves as a publicly editable and open-content encyclopaedia of internal control genes and accordingly bears broad utility for reliable RT-qPCR normalization and gene expression characterization in both model and non-model organisms. � The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

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Accessibility

Rate of accessibility:
HTTP status codeDate requested
200 OK2018-01-16
200 OK2018-01-12
200 OK2018-01-09
200 OK2018-01-05
200 OK2018-01-02
200 OK2017-12-29
200 OK2017-12-26
200 OK2017-12-22
200 OK2017-12-19
200 OK2017-12-15
200 OK2017-12-12
200 OK2017-12-08
200 OK2017-12-05
200 OK2017-12-01
200 OK2017-11-28
200 OK2017-11-24
200 OK2017-11-21
200 OK2017-11-17
200 OK2017-11-14
200 OK2017-11-10
200 OK2017-11-07

Tags

Metadata
Animal Bacteria Fungus Plant
RT-qPCR normalization

Record metadata

  • Created on: 2017-11-05
  • Curated by:
    • Lin Liu [2017-11-06]
    • Lin Liu [2017-11-05]
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